| Equations 1 and 2 use the the spectral profile
overlaps among the components listed.
| Eq1 |
Excitation Throughput (TEX) |
TEX = Fl. dye ex * Ex filt * lamp output
(or laser line)*1-DM |
| Eq2. |
Emission Throughput (TEM) |
TEM = Fl. dye em * Em filt * DM |
| Eq3. |
Instrument Efficiency (EI) |
EI = TEX * TEM |
| Eq4. |
Brightness Index (IB) |
IB= (Ec * QY)/1000 |
| Eq5. |
Theoretical Output (TO)* |
TO = EI * IB |
*If either QY or Ec, or both, are not available,
a value of “1” is used for IB in the
TO calculation.
DM = dichroic mirror
QY = fluorescent dye quantum yield
Ec = fluorescent dye Extinction coefficient
Ex max = wavelength at Excitation maximum
Em max = wavelength at Emission maximum
Stokes Shift = Em max – Ex max
|
