Microinjection Transformation: Gene Therapy for Worms! C. elegans researchers can introduce genes into worms by injecting DNA directly into the tissue that will give rise to the worms' egg cells. In many cases, the inserted genes function normally, giving researchers a powerful tool for examining their role in the worm. Our lab has used this technique to introduce isolated fragments of worm DNA into our spermatogenesis mutants. In some cases, the introduced DNA reverses the sterility of the mutant worm, suggesting that the DNA contains the normal copy of the gene that is disrupted in the mutant. With further molecular analysis of the DNA, we can precisely identify and characterize the gene, an important step in understanding its role in spermatogenesis. Want to learn more? watch a video of a worm being microinjected! protocols for microinjection transformation two worm transformation gurus: Dr. Andrew Fire Dr. Craig Mello some key references: Mello and Fire. (1995) Mello, et al. (1991) Kelly, et al. (1997) Jefferson, et al. (1987) Stinchcomb, et al. (1985)   research | gallery http://www.mcb.arizona.edu/wardlab/transformation.html All Contents Copyright © 2000. All rights reserved. Last Modified: June 30, 1998 Paul Muhlrad pmuhlrad@u.arizona.edu